Ferruginous nucleins and process of making same.



Nrrnn PATENT FFTQE.

EMIL SOHMOLL, OF BASLE, SWITZERLAND, ASSIGNOR TO BASLE CHEMICAL WORKS, OF SAME PLACE.

FERRUGINOUS NUCLEINS AND PROCESS OF MAKING SAME.

STPECIFICATION formingpart of Letters Patent No. 668,460, dated February 19, 1901.

Application filed September 10, 1900- Serial No. 29,510. (No specimens.)

To all whom, it may concern:

Be it known that I, EMIL SOHMOLL, chemist, doctor ofmedicine, a citizen of the Swiss Republic, and a resident of Basle, Switzerland,

5 have invented new and useful Improvements in the Manufacture of Ferrnginous Nucleins, of which the following isa clear and complete specification.

I have found that by cultivating yeast in [O ferruginous nutrient material the yeast takes up or absorbs part of the iron. From the yeast thus treated ferruginous nucleo-albumins may be isolated by artificial digestion. The nucleo-albumins thus obtained contain the iron only partly in stable organic combination-t'. 6., in a form from which the iron can be precipitated only after some time by a small quantity of ammonium sulfid. By treating these nucleo-albumins with a hot diluted solution of sodium carbonate a preparation is obtained from which all the iron can be precipitated only by a prolonged action of ammonium sulfidi. e., in this compound the iron is contained in a masked form analogous to that of the ferruginous nucleins contained in food. Example 1: Ten liters of fresh yeast containing dry matter amounting to about twenty per cent. of this volume are mixed with about one hundred to five hundred grams of ferric chlorid and fivehundred to five thousand grams of grape-sugar, and the mixture is digested with agitation for about five days. The liquid is then filtered oft and the solid residue stirred with ten liters of artificial gastric juice, at 37 centigrade, until the albumins of the cells are digested and only the grains of yeast are visible under the microscope. This lasts from ten to twelve hours. 40 The solid residue of the digestion is filtered off, boiled with a diluted solution of sodium carbonate, and then washed with hydrochloric acid of 0.3-per-cent. strength until no iron can be detected in the washings. The product is then dried and powdered.

Example II: A nutritious broth is mixed with 0.5 per cent. of citrate of iron and a sufficient quantity of sodium carbonate to dissolve the precipitate produced by the citrate of iron. The whole is then sterilized. The

solution is next inoculated with a pure culture of a species of yeast-for example, saccharomyces ctpiculatusuntil a sufficient quantity of the culture has been developed. The latter is then filtered, digested with artificial gastric juice, and treated further as in Example I.

The ferruginous nuclein obtained according to the foregoing examples is a yellowbrown powder insoluble in water, alcohol, and ether. In sodium carbonate and sodalye it is partly soluble when left standing for some time. This ferruginous nuclein shows a formation of iron sulfur only after being left to stand for some time with a small qnantity of ammonium sulfid. Larger quantities of this reagent precipitate iron sulfur after a shorter time. Alcoholic hydrochloric acid (ten volumes per cent. hydrochloric acid and ninety volumes per cent. alcohol of ninety-six-per-cent. strength) or 0.3 to 3 per cent. dilute hydrochloric acid does not separate iron, by which fact the ferruginous. nuclein can pass the stomach withoutbeing decomposed. When boiled with hydrochloric acid, it assumes a light-yellow color. If this solution then be made alkaline with NaOH, the ferruginous nuclein is again precipitated in large flakes. The ferruginous nuclein produced contains from one to one and fourtenths per cent. of iron and from three to four per cent. of phosphorus.

The ferruginous nuclein is used for persons as medicine as a remedy for chloro'sis and anemia in doses of 0.5 to one gram, to be taken three times a day.

What I claim is- 1. The herein-described process for the manufacture of ferruginous nucleins, which consists in first cultivating yeast in ferrngi- 9o nous nutrient material, then digesting the yeast thus obtained, then boiling the solid residue from the digestion with a dilute solution of sodium carbonate, and finally washing the said residue with dilute hydrochloric acid.

2. As a new article of manufacture, the herein-described ferruginous nuclein derived from yeast, the same containing phosphorus and from one to one and four-tenths per cent. I00

of iron in stable orgenie combination, end my name, this 27th day of August, 1900, in the being a yellow-brown powder, insoluble in presence of two subscribing witnesses. Water, alcohol and ether partly soluble in a sodium-carbonate solutioh and soda-lye when EMIL SOHMOLL' 5 left standing for some time, and not decom Witnesses:

posable in the stomach. GEO. GIFFORD,

In witness whereof I have hereunto signed AMAN D BITTER. 

